Cytotoxicity Comparison of a Calcium Silicate-Based Resin Cement versus Conventional Self-Adhesive Resin Cement and a Resin-Modified Glass Ionomer: Cell Viability Analysis

Objective: To compare the cytotoxicity level of a new calcium silicate-based resin cement (TheraCem) with two commonly used cements, including conventional self-adhesive (Panavia SA) and resinmodified glass ionomer (FujiCem2), on human gingival fibroblast cells after 24 48 hours. Material Methods: Twelve discs each type were fabricated. The extract disks was made by incubating them in cell medium. Human cultured exposed to extracts for h h. MTT assay performed optical density viability rates calculated spectrophotometer device at 570 nm. Data analyzed using ANOVA Tukey HSD tests. Results: hours as follows: TheraCem: 89.24% 85.46%, Panavia SA: 49.51% 46.57% FujiCem2: 50.63% 47.36%. TheraCem represented highest rate. However, no significant difference noted between SA FujiCem2. Time had effect viability. Conclusion: exhibited best results among three tested cements considered non-toxic. FujiCem2 not significantly different regarding cements.